The role of NADPH Oxidase 4 in Iraqi Osteoporosis Patients

Abstract

Background: Osteoporosis is a common condition that occurs in bone leading to the loss of bone mass. It is caused by increased osteoclast activity which is differentiated by low bone density measured by dual-energy X-ray absorptiometry (DEXA).Objective: This study aimed to estimate the levels of NADPH oxidase (NOX4), Alkaline phosphatase, and vitamin D in Iraqi osteoporosis patients diagnosed by  DEXA. Methods: A total of 88 volunteers were split into two groups for this investigation. There were 58 patients in group 1 and 30 controls in group 2 , the samples were taken from both sexes of range age (20-60) years, the levels of Alkaline phosphatase (ALP), Vitamin D, and NOX 4 were evaluated. NADPH oxidase 4  levels were measured by enzyme-linked immunosorbent assay (ELISA), Alkaline phosphates levels were measured using a spectrophotometer, and the concentration of vitamin D was measured by electrochemiluminescence binding assay using Cobas e 411. Whereas the T-score was measured using DEXA.Results: The results of NADPH oxidase 4, AlP, and vitamin D did not show any significant change (P>0.05) when compared osteoporosis group to the control group. Whereas T score showed a significant variance when contrasting between the studied groups (P<0.05). Conclusion: according to the presented results  NADPH oxidase 4 is not affected by osteoporosis.