Identification of MRSA(methicillin resistant Staphylococcus aureus) by mecA gene

Marwa  raad; Ameen Haider  Ahmed; Fakhri  Ahmed3

doi:10.55145/ajbms.2022.1.2.004

Authors

Marwa raad Al Salam University college. Department of optometry, Iraq
Ameen Haider Ahmed Al Salam University college. Department of Medical Laboratory Technique Department, Iraq
Fakhri Ahmed3 Al Salam University college. Department of Medical Laboratory Technique Department, Iraq

DOI:

https://doi.org/10.55145/ajbms.2022.1.2.004

Abstract

Staphylococcus aureus is a bacterium that may be found in the skin and upper respiratory system. Although S.aureus is a normal flora, it has evolved into an opportunistic pathogen that causes numerous infections that are difficult to cure due to the presence of virulence genes. The goal of this work is to identify MRSA in bacterial isolates using the mecA gene. In the current investigation, 213 samples were gathered from various clinical sources from patients hospitalized to various hospitals in Baghdad. The isolates were identified as S.aureus using normal bacteriological methods and verified using a molecular approach based on the nuc gene. Due to the many species of staphylococci that were expressed for varying resistance levels, the conventional approach was the best way for finding Methicillin resistant gene in S. aureus (mecA). Finally, mecAgene was found to be present in 76.67 % of bacterial isolates.