Eucalyptus extract and its synergistic effect on bacteria causing urinary tract infections in Diyala province

Authors

  • Duaa Adnan Kadhum Department of Medical Laboratories Techniques, Baqubah Technical Institute, Middle Technical University, Iraq.
  • Entisar Mahdi Hamad Middle Technical University, Baqubah Technical Institute, Department of Nursing Technologies, Iraq.
  • Shahrazad ahmed Khalaf University of Diyala, college of science, department of Biotechnology, Iraq.
  • Eman A. Muhsin Ministry of science and technology, Environment, Water and Renewable Energy Directorate- Baghdad- Iraq.

DOI:

https://doi.org/10.55145/ajbms.2024.03.01.010

Keywords:

Bacteria, Eucalptus, UTI

Abstract

To assess the impact of E. camaldulensis extract against bacteria that are isolated from urine sample from UTI patient in Baquba city Then mix this plant with antibiotics to see its effect. In addition to examine those pathogens bacteriologically. The sensitivity of the isolates to (13) antibiotics was tested, and the bacterial isolates showed a variation in the rates of their resistance to these antibiotics. The results showed that the antibiotic Imipenem had the most effect on the bacterial isolates under study, while the bacterial isolates showed high resistance to the rest of the antibiotics. The minimum inhibitory concentration (MIC) was determined for 3 antibiotics, namely, Gentamycin, Amikacin and Amoxicillin. These values for the antibiotics ranged between <4-1024, <4-1024 <<512-1024) mcg/ml, respectively. The Ethanolic Extract of Eucalyptus Exhibit highest antibacterial effect on S.areus on (o.62 g/1.5 ml and 0.31g/750ml). Lower respectively antibacterial effect on Proteus and Pseudomonas but no antibacterial effect against Klebsiella. Bacterial isolates were selected on the basis of their most frequent urinary tract infections and their resistance to the effect of antibiotics (K. pneumoniae, P. aeruginosa and S. aureus). The amount of the mixed effect was determined by mixing Eucalyptus with three antibiotics, namely Amikacin and Gentamycin Amoxicillin. The minimum inhibitory concentration of the isolates under study was compared before and after mixing the antibiotics. The effect of mixing was a synergistic effect for all bacterial isolates. On the other hand, the synergistic effect was clear on the gram-positive isolates of S. aureus between eucalyptus and beta-lactams (and Amoxicillin).

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Published

2024-01-20

How to Cite

Kadhum, D. A., Hamad, E. M., Khalaf, S. ahmed, & Muhsin, E. A. (2024). Eucalyptus extract and its synergistic effect on bacteria causing urinary tract infections in Diyala province. Al-Salam Journal for Medical Science, 3(1), 59–67. https://doi.org/10.55145/ajbms.2024.03.01.010

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